Industrial application of impurity flocculation to streamline antibody purification processes
نویسندگان
چکیده
2015 Flocculation technologies offer significant benefits to industrial mammalian cell culture processes, including increased clarification efficiency, impurity removal and process simplification. In this paper, the application and performance of flocculation technologies employed in the harvest process of monoclonal antibody Chinese hamster ovary cell culture are reviewed. Attention has been placed on technologies enhancing the removal of cells, cellular debris and reduction in impurities while maintaining the antibody in the product stream. Many flocculants are systematically evaluated with respect to their mechanism of action, impact on downstream processing and product quality, and potential disadvantages. Practical considerations and future directions for application of flocculation in antibody manufacturing are discussed.
منابع مشابه
Development of a novel and efficient cell culture flocculation process using a stimulus responsive polymer to streamline antibody purification processes
Recent advances in mammalian cell culture processes have significantly increased product titers, but have also resulted in substantial increases in cell density and cellular debris as well as process and product related impurities. As such, with improvements in titer, corresponding improvements in downstream processing are essential. In this study we have developed an alternative antibody harve...
متن کاملMonoclonal antibody purification using cationic polyelectrolytes: an alternative to column chromatography.
The potential of cationic polyelectrolytes to precipitate host cell and process related impurities was investigated, to replace one or more chromatography steps in monoclonal antibody purification. The impact of antibody isoelectric point, solution properties (pH and ionic strength), and polyelectrolyte properties (structure, molecular weight and pK(a)) on the degree of precipitation was studie...
متن کاملThe Application of Cluster-Surfactant Flocculants in the Advanced Treatment of Coking Industrial Wastewater Reclamation
Coking wastewater from the process of coking, coal gas purification and the coking products refine, whose ingredients are complicated and inconstant, is hard to meet the standard of reclaimed water used for industrial water by secondary treatment. In view of this situation, the advanced treatment technique of coking wastewater was desired. The innovatory flocculation treatment was used to regen...
متن کاملPurification of human anti-erythropoietin polyclonal antibodies by precipitation and chromatography as an optimized method with potential application in vaccine studies
Introduction: Polyclonal antibodies are required to be affinity purified. Improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. Two common techniques for purifying proteins is salt precipitation and chromatography purification. Our work fo...
متن کامل